| 名稱 | SRE-Luc/HEK293 |
| 型號(hào) | CBP74030 |
| 報(bào)價(jià) | 50000 |
| 特點(diǎn) | SRE-Luc/HEK293，凍存條件：90% FBS+10% DMSO； |
產(chǎn)品搜索
相關(guān)文章
- 喝熱茶或使食管癌風(fēng)險(xiǎn)增加5倍
- 證據(jù)：鍛煉或許并不是控制體重的關(guān)鍵
- 關(guān)于sigma試劑的使用時(shí)長(zhǎng)相關(guān)說(shuō)明
- 肺癌靶向治療：腫瘤不進(jìn)展時(shí)間超2年
- 一箭雙雕——IL-4R靶點(diǎn)和IL-4/IL-13雙重阻斷藥篩細(xì)胞模型
- IL36靶點(diǎn)藥物細(xì)胞篩選模型藥
- 腫瘤細(xì)胞培養(yǎng)實(shí)驗(yàn)的主要用途
- CCR8 | 潛在重磅腫瘤免疫治療靶點(diǎn)
- TIGIT&PVRIG雙靶點(diǎn)細(xì)胞篩選模型
- 重磅！科學(xué)家成功利用免疫細(xì)胞運(yùn)輸抗癌藥物至腫瘤靶點(diǎn)
聯(lián)系我們
聯(lián)系人：蔣經(jīng)理
電話：4008750250
號(hào)碼：
手機(jī)：18066071954
地址：南京市棲霞區(qū)緯地路9號(hào)
Email： zhangxiangwen@cobioer.com
電話：4008750250
號(hào)碼：
手機(jī)：18066071954
地址：南京市棲霞區(qū)緯地路9號(hào)
Email： zhangxiangwen@cobioer.com
產(chǎn)品展示 / PRODUCTS
藥靶細(xì)胞株 > 腫瘤免疫細(xì)胞株 > CBP74030SRE-Luc/HEK293
- 詳細(xì)內(nèi)容
| CBP74030 | |
| I. Background | |
| The MAPK/ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylate and activate ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The TCFs (Ternary Complex Factors), including Elk1, are among the best-characterized transcription factor substrates of ERK. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes. | |
| II. Description | |
| The SRE Reporter -HEK293 cell line is designed for monitoring the activity of the MAPK/ERK signaling pathway. The SRE Reporter -HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line. | |
| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | SRE-Luciferase |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | SRE cell line, MAPK reporter, ERK reporter |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+200ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
| |
Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose Response of Recombinant Human EGF in SRE/Luc HEK293 Cells (C5C16). | |
會(huì)員_a.png)