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藥靶細胞株 > 腫瘤免疫細胞株 > CBP74109hIL2/IL15 Dual Effector (mCD25/hCD122mCD132) Repor

hIL2/IL15 Dual Effector (mCD25/hCD122mCD132) Repor
名稱 hIL2/IL15 Dual Effector (mCD25/hCD122mCD132) Repor
型號 CBP74109
報價 200000
特點 hIL2/IL15 Dual Effector (mCD25/hCD122mCD132) Reporter Cell,凍存條件:90% FBS+10% DMSO;
  • 詳細內容
CBP74109
I. Background

The particles contain a firefly luciferase gene driven by the human IL-2 promoter (Figure 1). After transduction, activation of the IL-2 signaling pathway in the target cells can be monitored by measuring the luciferase activity.

IL-15 has been shown to be produced by human fetal astrocytes and microglial cells in response to IL-1beta, IFN-gamma, or TNF- alpha and may thus play a role in T-cell mediated immune responses in the human central nervous system. IL-15 stimulates proliferation of the established T-cell line CTLL-2, CD8(+) memory T- cells require IL-15 for proliferation. IL-15 is also able to induce generation of cytolytic cells and LAK cells activity in vitro. IL-15 appears to function as a specific maturation factor for NK-cells and can mimic the bone marrow microenvironment in vitro, which is required for NK- cells to acquire lytic functions. IL-15 induces proliferation of mast cells in the absence of functional IL-2 receptor components and utilizes a receptor of 60-65 kDa that is distinct from known components of T-cell IL-15 receptors. The IL-15 receptor on mast cells recruits JAK2 and STAT5, instead of JAK1, JAK3, and STAT3 and STAT5 that are activated in T-cells. The alpha subunit of the IL-15 receptor (IL-15RA) is structurally related to the alpha subunit of the IL-2 receptor and confers high affinity binding of IL-15 to its receptor. IL-15 binds to the beta and gamma chains of the IL2 receptor, which are known to be required for ligand internalization and signal transduction.

 
II. Introduction
Expressed gene:IL2R、IL15R
Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):IL15, AI503618, IL-15, interleukin 15, IL15, Il15
Freeze Medium:90% FBS+10% DMSO
Culture Medium:RPMI-1640+10%FBS+10ng/ml IL-3+600ug/ml hygromycin+2ug/ml puromycin
Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):Functional(Report Gene) Assay
 
III. Representative Data

CBP74109 圖1.jpg

Figure 1. Dose Response of Recombinant HumanlL15 in hlL2IL15 Dual Effector(mCD25hCD122mCD132) Reporter Cells.

CBP74109 圖2.jpg

Figure 2. Dose Response of Recombinant Human lL2 in hIL2/IL15 Dual Effector (mCD25hCD122mCD132) Reporter Cells (C31).

 

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