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藥靶細胞株 > 腫瘤免疫細胞株 > CBP74163PD-1/OX40 Dual Effector Reporter Cell IO細胞株

PD-1/OX40 Dual Effector Reporter Cell IO細胞株
名稱 PD-1/OX40 Dual Effector Reporter Cell IO細胞株
型號 CBP74163
報價
特點 PD-1/OX40 Dual Effector Reporter Cell
  • 詳細內(nèi)容
CBP74163
I. Background

The binding of Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T cells, to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. The PD-1 ligands are found on most cancers, and PD-1:PD-L1/2 interaction inhibits T cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.

OX40 (CD134) is a co-stimulatory receptor expressed on the surface of CD4+ and CD8+ T cells 24 to 48 hours after activation. Binding of OX40 to its ligand, OX40L (CD252), present on dendritic cells, potentiates T cell survival and increases cytokine production. OX40 has been shown to activate NF-κB-mediated memory cell generation through its interaction with adaptor proteins TRAF2 and TRAF5. OX40 has a critical role in the maintenance of an immune response beyond the first few days and onwards to a memory response.

 
II. Introduction
Expressed gene:PD-1/OX40
Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:90% FBS+10% DMSO
Culture Medium:RPMI-1640+10%FBS+1ug/ml puromycin+800ug/ml hygromycin+5ug/ml blasticidin
Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):Functional(Report Gene) Assay
 
III. Representative Data

CBP74163 流式1.png

Figure 1. Recombinant PD-1/OX40 Dual Effector Reporter Cell constitutively expressing OX40.

CBP74163 流式2.png

Figure 2. Recombinant PD-1/OX40 Dual Effector Reporter Cell constitutively expressing PD-1.

CBP74163、CBP74164 數(shù)據(jù)圖.png

Figure 3. Dose Response of Blocking Antibodies in PD-1/OX40 Dual Effector Reporter Cells (C22) With PD-L1 aAPC Cells.

 

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