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藥靶細胞株 > 腫瘤免疫細胞株 > CBP74106ADCP Bioassay Effector Cell FcγRIIa (H variant)-NF

ADCP Bioassay Effector Cell FcγRIIa (H variant)-NF
名稱 ADCP Bioassay Effector Cell FcγRIIa (H variant)-NF
型號 CBP74106
報價
特點 ADCP Bioassay Effector Cell FcγRIIa (H variant)-NFAT/Jurkat,母細胞:Jurkat,凍存條件:90% FBS+10% DMSO
  • 詳細內(nèi)容


CBP74106
I. Background

Antibody-dependent cell-mediated phagocytosis (ADCP) is one of the important mechanisms of action for antibody drug development. FcγRIIa is the predominant Fcγ receptor involved in the ADCP process. FcγRIIa is expressed in myeloid effector cells, including macrophages and neutrophils, where it plays a role in the activation of these effector cells. Several clinical studies have studied the correlation of a FcγRIIa polymorphism (R131H) and the response to IgG1 subclass monoclonal antibodies (mAbs) such as rituximab. Engineered amino-acid substitutions in Fc-mAbs have been developed to enhance the mAb-mediated phagocytosis of tumor cells by macrophages.

II. Description

Recombinant Jurkat T cell expressing a firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIa, Histidine variant.

III. Introduction
Host Cell:Jurkat
Expressed gene:FcγRIIa (H variant)-NFAT
Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:90% FBS+10% DMSO
Culture Medium:RPMI-1640+10%FBS+1ug/ml puromycin+400ug/ml hygromycin
Storage:Liquid nitrogen
Application(s):Functional(Report Gene) Assay
IV. Description of Host Cell Line
Organism:Human
Tissue:Peripheral blood
Disease: Childhood T acute lymphoblastic leukemia
Morphology: Lymphoblast
Growth Properties: Suspension
Ⅴ. Representative Data

CBP74106.png

Figure 1:Dose response of Rituximab in ADCP Bioassay Effector Cell FcyRlla (H variant) /NFAT Reporter-Jurkat (C2),the EC50 was 21.5ng/ml.

 





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